Partitioning and purification of the cellulolytic complex produced by Aspergillus japonicus URM5620 using PEG–citrate in an aqueous two-phase system
作者： Polyanna N Herculano , Tatiana S Porto , Marilia HC Maciel , Keila A Moreira , Cristina M Souza-Motta
DOI: 10.1016/J.FLUID.2012.08.008
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摘要: Abstract The partitioning and purification of cellulases from the cellulolytic complexes produced by Aspergillus japonicus URM5620 were investigated in aqueous two-phase systems (ATPS). A factorial design model (2 4 ) was used to evaluate influence polyethylene glycol (PEG) molar mass (1000–8000 g/mol), PEG concentration (20.0–24.0%, w/w), sodium citrate (15–20%, w/w) pH (6.0–8.0) on differential complex composed β-glucosidase (βG), endoglucanase (CMCase) total cellulase (FPase). βG preferentially partitioned salt phase; however, when simultaneously increased, partition coefficient increased ( K = 3.0). For βG, 20% (w/w) 8000 (g/mol) 10% at 8.0 best results; approximately 2.9, activity yield 77%, factor 1.9. FPase CMCase top phase. highest values (2.9 3.0) obtained phase consisted 1000 (g/mol). for (2.67%) with 24% 15% 8.0. However, (64.8) observed CMCase, (1.64%) (45.9) 22% 3350 17.5% 7.0. PEG/sodium system resulted separations characteristic complexes, where partitions This process provides an efficient attractive increase factor.
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