Characterization of in vitro oral bacterial biofilms by traditional and molecular methods.

摘要: The aim of this study was to compare culture-based bacterial isolation methods with direct amplification and cloning 16S rRNA genes from oral biofilms grown in an vitro model. model used a constant depth film fermentor which inoculated pooled human saliva. use culture techniques resulted the identification 36 different species saliva inoculum biofilms. Of these, only five were detected solely by molecular methods. Three taxa which, according databases, unidentified. Using detection, differences number observed found using gene primers numbers PCR cycles. We have shown that microcosm supragingival plaque consisted community most members could be cultivated on laboratory media.