Isolation of bacteriophages from the oral cavity

作者: G. Hitch , J. Pratten , P.W. Taylor

DOI: 10.1111/J.1472-765X.2004.01565.X

关键词:

摘要: Aims: To isolate bacteriophages lytic for oral pathogens from human saliva, dental plaque and mature biofilms constituted saliva-derived bacteria.Methods Results: Saliva samples healthy volunteers patients with gingivitis periodontitis were examined the presence of bacteriophage using a panel bacteria isolated samples. Samples also enriched static culture techniques biofilms. A limited number contained particles that visualized electron microscopy. Cultures yielded phage infecting non-oral (Proteus mirabilis) but no specific recognized found. Some micro-organisms microflora elaborated antibacterial substances inhibited growth other residents cavity.Conclusions: Unlike ecosystems, composition cavity does not appear to be heavily influenced by interactions between their hosts.Significance Impact Study: Bacteriophage control infections may need obtained sources. Antibacterial derived some members warrant investigation as potential antibiotics.

参考文章(22)
Allan Campbell, The future of bacteriophage biology Nature Reviews Genetics. ,vol. 4, pp. 471- 477 ,(2003) , 10.1038/NRG1089
C A Tylenda, C Calvert, P E Kolenbrander, A Tylenda, Isolation of Actinomyces bacteriophage from human dental plaque. Infection and Immunity. ,vol. 49, pp. 1- 6 ,(1985) , 10.1128/IAI.49.1.1-6.1985
J. Pratten, M. Wilson, Antimicrobial susceptibility and composition of microcosm dental plaques supplemented with sucrose Antimicrobial Agents and Chemotherapy. ,vol. 43, pp. 1595- 1599 ,(1999) , 10.1128/AAC.43.7.1595
A L Delisle, J A Donkersloot, P E Kolenbrander, C A Tylenda, Use of lytic bacteriophage for Actinomyces viscosus T14V as a probe for cell surface components mediating intergeneric coaggregation. Infection and Immunity. ,vol. 56, pp. 54- 59 ,(1988) , 10.1128/IAI.56.1.54-59.1988
H. Sandmeier, A.J. Winkelhoff, K. Bar, E. Ankli, M. Maeder, J. Meyer, Temperate bacteriophages are common among Actinobacillus actinomycetemcomitans isolates from periodontal pockets Journal of Periodontal Research. ,vol. 30, pp. 418- 425 ,(1995) , 10.1111/J.1600-0765.1995.TB01296.X
Allan L. Delisle, Christine A. Rostkowski, Lytic bacteriophages ofStreptococcus mutans. Current Microbiology. ,vol. 27, pp. 163- 167 ,(1993) , 10.1007/BF01576015
H. Hiroki, J. Shiiki, A. Handa, M. Totsuka, O. Nakamura, Isolation of bacteriophages specific for the genus Veillonella. Archives of Oral Biology. ,vol. 21, pp. 215- 217 ,(1976) , 10.1016/0003-9969(76)90132-1
Charles E. Meyers, Eugene L. Walter, Lynn B. Green, Isolation of a Bacteriophage Specific for a Lactobacillus Casei From Human Oral Material Journal of Dental Research. ,vol. 37, pp. 175- 178 ,(1958) , 10.1177/00220345580370011201
J. Pratten, M. Wilson, D. A. Spratt, Characterization of in vitro oral bacterial biofilms by traditional and molecular methods. Oral Microbiology and Immunology. ,vol. 18, pp. 45- 49 ,(2003) , 10.1034/J.1399-302X.2003.180107.X