Biology and genome sequence of Streptococcus mutans phage M102AD

摘要: M102AD is the new designation for a Streptococcus mutans phage described in 1993 as M102. This change was necessitated by genome analysis of another S. named M102, which revealed differences from sequence reported here. Additional host range analyses confirmed that infects only few serotype c strains. Phage adsorbed very slowly to its host, and it cannot adsorb e f strains mutans. adsorption blocked c-specific antiserum. also equally well heat-treated trypsin-treated cells, suggesting carbohydrate receptors. Saliva polysaccharide production did not inhibit plaque formation. The this siphophage consisted linear, double-stranded, 30,664-bp DNA molecule, with GC content 39.6%. Analysis extremities indicated presence 3′-overhang cos site 11 nucleotides long. Bioinformatic identified 40 open reading frames, all same orientation. No lysogeny-related genes were found, indicating strictly virulent. obvious virulence factor gene candidates found. Twelve proteins virion structure mass spectrometry. Comparative genomic close relationship between phages M102 thermophilus phages. study highlights importance conducting research biological materials obtained recognized microbial collections.