Characterization of the Systemic Loss of Dendritic Cells in Murine Lymph Nodes During Polymicrobial Sepsis

摘要: Dendritic cells (DCs) play a key role in critical illness and are depleted spleens from septic patients mice. To date, few studies have characterized the systemic effect of sepsis on DC populations lymphoid tissues. We analyzed phenotype DCs Th present local (mesenteric) distant (inguinal popliteal) lymph nodes mice with induced polymicrobial (cecal ligation puncture). Flow cytometry immunohistochemical staining demonstrated that there was significant (mesenteric nodes) partial (inguinal, but not popliteal loss mice, this process associated increased apoptosis. This sepsis-induced occurred after CD3(+)CD4(+) T cell activation nodes, preceded by any sustained increase their maturation status. In addition, no preferential either mature/activated (MHCII(high)/CD86(high)) or immature (MHCII(low)/CD86(low)) during sepsis. However, CD8(+) nodes. The tissue, particularly lymphoid-derived DCs, may contribute to alterations acquired immune status frequently accompany