Prominin-1-Specific Binding Peptide-Modified Apoferritin Nanoparticle Carrying Irinotecan as a Novel Radiosensitizer for Colorectal Cancer Stem-Like Cells

摘要: Resistance of cancer stem cells to radiotherapy remains a major obstacle successful management. Prominin-1 (PROM1) is cell marker. Nanoparticle (NP) chemotherapeutics preferentially accumulate in tumors and are able target stem-like through cell-specific ligands, making them uniquely suited as radiosensitizers for chemoradiation therapy. Using biocompatible apoferritin NP, PROM1-targeted NP carrying irinotecan (PROM1-NP) engineered. The synergistic effect the irradiation evaluated PROM1-overexpressing HCT-116 colorectal lines vitro vivo. PROM1-NP has size 17.2 ± 0.2 nm surface charge −13.5 mV. It demonstrates higher intracellular uptake than nontargeted or alone. Treatment with PROM1-NPs decreases proliferation dose- time-dependent manner. In radiosensitization reveals that significantly more effective radiosensitizer irinotecan. tumor xenograft growth markedly slower following treatment plus irradiation, suggesting This study provides first preclinical evidence effectiveness formulation radiosensitizer.