Cytotoxic sensitivity to tumor necrosis factor-α in PC3 and LNCaP prostatic cancer cells is regulated by extracellular levels of SGP-2 (clusterin)

摘要: BACKGROUND SGP-2 is a ubiquitous secreted glycoprotein that prevents cellular apoptosis. This study was carried out to determine the extracellular action of SGP-2 in model tumor necrosis factor-α (TNF)-induced cytotoxicity using two human prostatic cancer lines, LNCaP and PC3. These lines were selected because cells are highly sensitive cytotoxic effect TNF, while PC3 resistant TNF at 24 hr. METHODS Cells cultured presence or absence (10 ng/ml). treated with varying concentrations exogenous SGP-2, antisera without SGP-2. Following 24-hr treatment, cultures assessed by counting cell number trypan blue exclusion assay. RESULTS Western blot analysis conditioned media revealed more than did LNCaP. The sensitivity reduced addition became when antibody added culture. on reversed culture. CONCLUSIONS These results suggest can act as an mediator anti-TNF-induced cytotoxicity. Prostate 39:87–93, 1999. © 1999 Wiley-Liss, Inc.