Differentiating astroglia in nervous tissue histogenesis/regeneration: Studies in a model system of regenerating peripheral nerve

摘要: The role of astroglia in nervous system histogenesis/regeneration (morphogenesis) was studied as a function cell age. effect inoculated at different maturation stages on axonal growth examined peripheral nerve regenerating model system. This consists rat sciatic stumps that regenerate through an empty silicone chamber (Lundborg et al.: Journal Neuropathology and Experimental Neurology 41:412–422, 1982). Rat astroglial populations grown culture were suspended either liquid (physiological solution) or solid (isotonic collagen gel) medium within the time surgery. Immature mature populations, ages corresponding to 9–69 postnatal days (P9–P69), inoculated, their neural analyzed by histological, immunocytochemical, ultrastructural techniques, 2–6 weeks later. Astroglial cells differentially modulated process regeneration, is cells' developmental stage. P19 older inhibited regeneration process, encapsulating axons glia-limitans-like structure. astrocytes (P9) did not seem interfere with process; outgrowth presence resembled comparable ones obtained Schwann cells. differential effects developing significantly changed inoculation media, e.g., solid, already pronounced 2 after transection. It suggested results study morphogenesis are changing differentiation. Adult downregulate growth; presumably, confine neurites designated structural functional boundaries.