Nucleotide sequence and expression of a Drosophila metallothionein.

作者: D Lastowski-Perry , E Otto , G Maroni

DOI: 10.1016/S0021-9258(18)89624-7

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摘要: A Drosophila melanogaster cDNA clone was isolated based on its more intense hybridization to RNA sequences from copper-fed larvae than control larval RNA. This showed strong mouse metallothionein I at reduced stringency. Its nucleotide sequence includes an open reading segment which codes for a 40-amino acid protein; this protein is identified as similarity the amino-terminal portion of mammalian and crab metalloproteins. The 10 cysteine residues present occur in five pairs near vicinal cysteines (Cys-X-Cys). hybridized 400-nucleotide polyadenylated whose presence cells alimentary canal stimulated by ingestion cadmium or copper; other tissues much lower levels. Mercury, silver, zinc induced lesser extent. level increased very soon after initiation metal treatment reached maximum approximately 36 h.

参考文章(23)
Monica Nordberg, Yutaka Kojima, Metallothionein and Other Low Molecular Weight Metal-Binding Proteins Experientia Supplementum. pp. 41- 124 ,(1979) , 10.1007/978-3-0348-6493-0_2
Joachim Messing, New M13 vectors for cloning. Methods in Enzymology. ,vol. 101, pp. 20- 78 ,(1983) , 10.1016/0076-6879(83)01005-8
D.M. Durnam, R.D. Palmiter, Transcriptional regulation of the mouse metallothionein-I gene by heavy metals. Journal of Biological Chemistry. ,vol. 256, pp. 5712- 5716 ,(1981) , 10.1016/S0021-9258(19)69264-1
K Lerch, D Ammer, R W Olafson, Crab metallothionein. Primary structures of metallothioneins 1 and 2. Journal of Biological Chemistry. ,vol. 257, pp. 2420- 2426 ,(1982) , 10.1016/S0021-9258(18)34940-8
G. Maroni, D. A. Adams, A. N. Wilton, C. C. Laurie-Ahlberg, GENETIC VARIATION IN THE EXPRESSION OF ADH IN DROSOPHILA MELANOGASTER Genetics. ,vol. 101, pp. 431- 446 ,(1982) , 10.1093/GENETICS/101.3-4.431
C.Thomas White, Stephen C. Hardies, Clyde A. Hutchison, Marshall H. Edgell, The diagonal-traverse homology search algorithm for locating similarities between two sequences Nucleic Acids Research. ,vol. 12, pp. 751- 766 ,(1984) , 10.1093/NAR/12.1PART2.751
D. A. Goldberg, Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 5794- 5798 ,(1980) , 10.1073/PNAS.77.10.5794
M. D. Biggin, T. J. Gibson, G. F. Hong, Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 3963- 3965 ,(1983) , 10.1073/PNAS.80.13.3963
Michael Karin, Alois Haslinger, Heidi Holtgreve, Robert I. Richards, Paul Krauter, Hannes M. Westphal, Miguel Beato, Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene. Nature. ,vol. 308, pp. 513- 519 ,(1984) , 10.1038/308513A0