作者: D Lastowski-Perry , E Otto , G Maroni
DOI: 10.1016/S0021-9258(18)89624-7
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摘要: A Drosophila melanogaster cDNA clone was isolated based on its more intense hybridization to RNA sequences from copper-fed larvae than control larval RNA. This showed strong mouse metallothionein I at reduced stringency. Its nucleotide sequence includes an open reading segment which codes for a 40-amino acid protein; this protein is identified as similarity the amino-terminal portion of mammalian and crab metalloproteins. The 10 cysteine residues present occur in five pairs near vicinal cysteines (Cys-X-Cys). hybridized 400-nucleotide polyadenylated whose presence cells alimentary canal stimulated by ingestion cadmium or copper; other tissues much lower levels. Mercury, silver, zinc induced lesser extent. level increased very soon after initiation metal treatment reached maximum approximately 36 h.