Novel defensin subfamily from spinach (Spinacia oleracea)

作者: Ana Segura , Manuel Moreno , Antonio Molina , Francisco Garcı́a-Olmedo

DOI: 10.1016/S0014-5793(98)01060-6

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摘要: Antimicrobial peptides (So-D1-7) were isolated from a crude cell wall preparation spinach leaves (Spinacia oleracea cv. Matador) and, judged their amino acid sequences, six of them (So-D2-7) represented novel structural subfamily plant defensins (group IV). Group-IV also functionally distinct those groups I–III. They active at concentrations <20 μM against Gram-positive (Clavibacter michiganensis) and Gram-negative (Ralstonia solanacearum) bacterial pathogens, as well fungi, such Fusarium culmorum, F. solani, Bipolaris maydis, Colletotrichum lagenarium. Fungal inhibition occurred without hyphal branching. preferentially distributed in the epidermal layer subepidermal region stems.

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