Inhibition of nitric oxide (NO•) production in murine macrophages by flavones

摘要: The effect of flavone (2-phenylbenzopyran-4-one) and three amino-substituted flavones on the production nitrite by murine activated peritoneal macrophages was studied in vitro. Activated obtained from mice pre-treated with concanavalin A (Con A) (in vivo), after exposure vitro to lipopolysaccharide (LPS) at a concentration 100 ng/ml, produced (20.3 ± 2.5 nmol/106 cells), as measured 24 hr Griess reaction. Stimulation inhibited NG-mono-methyl-L-arginine, suggesting that formed via nitric oxide (NO•) product metabolism arginine. aminoflavones (20–100 μM). 3′-amino-4′-hydroxyflavone most potent inhibitor production. Genistein (5,7-dihydroxy-3-(4-hydroxy-phenyl)-4H-1-ben-zopyran-4-one) also nitrite, mechanism appears not involve protein tyrosine kinases. These results suggest can modulate immune responses inflammatory reactions controlling oxide.