作者: Rawil F. Fakhrullin , Alsu I. Zamaleeva , Mikhail V. Morozov , Diana I. Tazetdinova , Farida K. Alimova
DOI: 10.1021/LA803871Z
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摘要: We report the layer-by-layer coating of living fungi cells (Saccharomyces cerevisiae and Trichoderma asperellum) with polyelectrolytes poly(allylamine hydrochloride)/sodium poly(styrene sulfonate) bovine serum albumin/DNA citrate-stabilized gold silver nanoparticles. It was found that nanoparticles were effectively incorporated between oppositely charged polyelectrolyte layers, modifying topography roughness cell walls. The formation large aggregates on walls encapsulated shown. preserved their viability shells soft enough to allow growth mycelium. surface-enhanced Raman scattering (SERS) used investigate biochemical environments immobilized surface T. asperellum conidia. SERS spectra from conidia indicate both interact different locations, nanoparticle-polyelectrolyte interaction is limited. approach described in this paper might have potential applications modification cells.