Voltammetric determination of epinephrine by White rot fungi (Phanerochaete chrysosporium ME446) cells based microbial biosensor.
作者： Erol Akyilmaz , Mehmet Turemis , İhsan Yasa
DOI: 10.1016/J.BIOS.2010.11.012
关键词:
摘要: Abstract The lyophilized biomass of White rot fungi ( Phanerochaete chrysosporium ME446) was immobilized in gelatine using glutaraldehyde crosslinking agent on a Pt working electrode. fungal cells retained their laccase activity under entrapped state. were used as source to develop amperometric epinephrine biosensor. catalytic action the biosensor released an epinephrinequinone result redox activity, thereby causing increase current. optimal conditions carried out at pH 4.5 (50 mM acetate buffer containing 100 mM K 3 Fe(CN) 6 ), and 20 °C. sensor response linear over range 5–100 μM epinephrine. detection limit found be 1.04 μM. In optimization characterization studies microbial some parameters such effect amount, percentage substrate specificity out. application biosensor, sensitive determination pharmaceutical ampules investigated.
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