IL-6 is a differentiation factor for M1 and WEHI-3B myeloid leukemic cells.

摘要: IL-6 has multiple biologic activities in different cell systems including both the ability to support proliferation and induce differentiation. We reported previously isolation functional expression of a mouse (mIL-6) cDNA clone derived from bone marrow stromal cells. In this paper, we show that mIL-6 is potent inducer terminal macrophage differentiation for myeloid leukemic line, M1. Addition cultures M1 cells rapidly inhibits their induces phagocytic activity morphologic changes characteristic mature macrophages. These phenotypic are accompanied at molecular level by decrease proto-oncogene c-myc mRNA accumulation increases Fc gamma R, proto-oncogenes c-fos c-fms (CSF-1R) expression. Furthermore, enhances R differentiation-responsive D+, but not unresponsive D- sublines myelomonocytic WEHI-3B Together with our previous observation stimulates colony formation normal progenitors, these results strongly suggest an important regulatory role growth