Comprehensive proteomic analysis of breast cancer cell membranes reveals unique proteins with potential roles in clinical cancer.

摘要: Proteins associated with cancer cell plasma membranes are rich in known drug and antibody targets as well other proteins to play key roles the abnormal signal transduction processes required for carcinogenesis. We describe here a proteomics process that comprehensively annotates protein content of breast tumor defines clinical relevance such proteins. Tumor-derived lines were used ensure an enrichment cell-specific membrane because it is difficult purify cells then obtain good preparations from material. Multiple different molecular pathologies represent heterogeneity cancer. Peptide tandem mass spectra searched against comprehensive data base containing conceptual derived many public bases including draft human genome sequences. This membrane-enriched proteome analysis created more than 500 line proteins, 27% which unknown function. The value our approach demonstrated by further detailed analyses three previously uncharacterized whose has been defined their unique expression profiles identification protein-binding partners elucidate potential functionality