Characterization of two amine oxidases from Aspergillus carbonarius AIU 205

作者: Asami Sugawara , Daisuke Matsui , Miwa Yamada , Yasuhisa Asano , Kimiyasu Isobe

DOI: 10.1016/J.JBIOSC.2014.10.023

关键词:

摘要: We have reported that Aspergillus carbonarius AIU 205, which was isolated by our group, produced three enzymes exhibiting oxidase activity for 4-aminobutanamide (4-ABAD) (J. Biosci. Bioeng., 117, 263–268, 2014). Among enzymes, characteristics of enzyme I been revealed, but those the other two not. In this study, we purified II and III, compared their with I. Enzymes III also oxidized aliphatic monoamines, aromatic amines, aminoalcohols. addition, both strongly inhibited carbonyl reagents specific inhibitors copper-containing amine oxidases. Thus, were classified into group (EC 1.4.3.6) along However, these differed from each in enzymatic, kinetic, physicochemical properties. The N-terminal amino acid sequences other; modified, similar to peroxisomal oxidases, oxidases genus . Therefore, concluded A. carbonarius 205 different types mycelia.

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