Comparative mapping in the pig: localization of 214 expressed sequence tags.
作者： Susanna Cirera , Claus B. Jørgensen , Milena Sawera , Terje Raudsepp , Bhanu P. Chowdhary
DOI: 10.1007/S00335-002-2242-Z
关键词:
摘要: In total, 214 ESTs (Expressed Sequence Tags) were assigned to the porcine gene map by using somatic cell hybrid mapping, radiation and FISH. The isolated from a small intestine cDNA library on basis of significant sequence identity with human annotated genes. 390 primer pairs designed primarily in 3′ UTR sequences. Overall, 58.6% successfully mapped this approach. 191 localizations are agreement comparative map, strongly indicating that these represent true orthologous remaining 23 provide new mapping data, which should be considered as preliminary until confirmed other studies. Our efforts contribution well for pig.
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