Control of Redox Balance by the Stringent Response Regulatory Protein Promotes Antioxidant Defenses of Salmonella

摘要: We report herein a critical role for the stringent response regulatory DnaK suppressor protein (DksA) in coordination of antioxidant defenses. DksA helps fine-tune expression glutathione biosynthetic genes and discrete steps pentose phosphate pathway tricarboxylic acid cycle that are associated with generation reducing power. Control NAD(P)H/NAD(P)+ redox balance by fuels downstream enzymatic systems nutritionally starving Salmonella. Conditional glucose-6-phosphate dehydrogenase-encoding gene zwf, shown here to be under control, increases both NADPH pool defenses dksA mutant The DksA-mediated boosts stationary phase bacteria. Not only does increase resistance Salmonella against hydrogen peroxide (H2O2), but it also promotes fitness this intracellular pathogen when exposed oxyradicals produced phagocyte oxidase an acute model infection. Given adjustment most bacteria undergoing nutritional deprivation, our findings raise possibility control central metabolic pathways maintains homeostasis essential phylogenetically diverse bacterial species.