Instrumentation and specimen preparation for electron beam X-ray microanalysis of frozen hydrated bulk specimens.
作者： Wolfgang Fuchs , Bernd Lindemann , Jobst D. Brombach , Walter Trösch
DOI: 10.1111/J.1365-2818.1978.TB01155.X
关键词:
摘要: SUMMARY A cooling chain for the handling of frozen hydrated bulk specimens is described. Use this method permits specimen to be kept fully hydrated. After quench freezing, transferred a freeze etch apparatus, fractured, carbon coated and onto precooled cold stage SEM by means an airlock. The examined in secondary electron mode analysed using energy dispersive X-ray analyser. midgut Chironomus thummi larvae frog skin epithelium were used test performance.
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