Aquifer Protist Response and the Potential for TCE Bioremediation with Burkholderia cepacia G4 PR1.

摘要: Bacterivorous protists have been recovered from pristine and contaminated aquifer environments, but the ecological role of these organisms in bioremediation strategies has not well defined. Burkholderia cepacia G4 PR1 constitutively expresses a toluene ortho-monooxygenase (tom) due to secondary transposition Tn5 transposable element trichloroethylene (TCE) degradative plasmid (TOM). Groundwater sediment potential site for TCE field demonstration were used laboratory microcosms test survival this organism. In nonsterile slurries, bacterium was eliminated logrithmic decay concomitant with an increase bacterivorous protists. A half-life organism calculated extinction coefficients increased logarithmically increasing inoculation density above 1 x 10(6) ml(-1). For densities below level, exponentially decreasing density. The lowest half-lives corresponded bacteria that stimulate response bacterivores. column system designed incorporate flow, repeated addition resulted buildup bacterivore populations reduced bacterium. Addition growth substrate eluent prolonged apparent mineralization TCE. results indicate significant predictable losses native bacterivores would occur within beyond treatment zone where be added aquifer, groundwater might possible nutrients.