GSK-J4-Mediated Transcriptomic Alterations in Differentiating Embryoid Bodies.

摘要: Histone-modifying enzymes are key players in the field of cellular differentiation. Here, we used GSK-J4 to profile important target genes that responsible for neural Embryoid bodies were treated with retinoic acid (10 μM) induce differentiation presence or absence GSK-J4. To GSKJ4-target genes, performed RNA sequencing both normal and demethylase-inhibited cells. A total 47 58 up- down-regulated, respectively, after exposure at a log2-fold-change cut-off value 1.2 (p-value < 0.05). Functional annotations all differentially expressed revealed significant number associated suppression proliferation, cell cycle progression induction death. We also identified an enrichment potent motifs selected expressed. Additionally, listed upstream transcriptional regulators genes. Our data indicate affects biology by inhibiting proliferation through These findings will expand current understanding histone-modifying enzymes, thereby promoting further investigations elucidate underlying mechanisms.