Influence of three-dimensional scaffold on the expression of osteogenic differentiation markers by human dermal fibroblasts.
作者： C HEE , M JONIKAS , S NICOLL
DOI: 10.1016/J.BIOMATERIALS.2005.07.004
关键词:
摘要: Abstract Current research in the field of tissue engineering utilizes biomaterial scaffolds, cells, and growth factors for creation a functional, biologically active tissue. This study examined effect two commercially available, three-dimensional ultraporous β -tricalcium phosphate ceramics ( -TCP, Vitoss) open-celled poly(lactic acid) foams (OPLA, Drilac), on osteogenic differentiation potential human dermal fibroblasts. Serum-free, chemically-defined medium containing metabolic factor 1 α ,25-dihydroxyvitamin D 3 was used to promote an phenotype these cells. Osteoblast assessed using PCR immunohistochemical methods detect gene protein expression osteoblast markers alkaline phosphatase, osteopontin, osteocalcin. Dermal fibroblasts cultured -TCP scaffolds with vitamin exhibited up-regulated compared cells OPLA scaffolds. These results suggest that Vitoss -TCP) seeded maintained are better suited bone applications than Drilac (OPLA) foams.
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