Proteomic and ecophysiological responses of soybean ( Glycine max L.) root nodules to Pb and hg stress

摘要: Lead (Pb) and mercury (Hg) are persistent hazardous metals in industrially polluted soils which can be toxic low quantities. Metal toxicity cause changes at cellular molecular level should studied for better understanding of tolerance mechanism plants. Soybean (Glycine max L.) is an important oilseed crop the world including India. Indian growing soybean often contaminated by Pb Hg. The aim this study was to explore how root nodule responds Hg through proteomic ecophysiological alterations order enhance metal stress. plants were exposed (30 ppm PbCl2) (0.5 ppm HgCl2) histological, histochemical, biochemical response N2-fixing symbiotic nodules. Both treatment increased oxidative stress leaves Chlorosis morphological/anatomical nodules observed. Activities ascorbate peroxidase, glutathione reductase catalase also modulated. Significant observed abundance 76 proteins influenced 33 (17 up 16 down) 43 (33 10 down), respectively. MS/MS ion search identified 55 functionally associated with numerous functions. Six crucial namely (CAT), allene oxide synthase (AOS), S-transferase (GST), calcineurin B like (CBL), calmodulin (CML) rapid alkalinisation factor (RAF) selected transcript estimation. qRT-PCR based real time expression exhibited a positive correlation proteomics except GST RAF. defence, development repair related proteins. An efficient modulation might lead metal-induced Although concentrations used cannot considered equimolar, yet seems induce more profile, higher damage both bacteroides anatomy.