Controlled rotation and vibration of patterned cell clusters using dielectrophoresis

摘要: The localized motion of cells within a cluster is an important feature living organisms and has been found to play roles in cell signaling, communication, migration, thus affecting processes such as proliferation, transcription, organogenesis. Current approaches for inducing dynamic movement into cells, however, focus predominantly on mechanical stimulation single affect integrity, and, more importantly, need complementary mechanism pattern cells. In this article, we demonstrate new strategy the large clusters, taking advantage dielectrophoresis. This based cellular spin resonance mechanism, but it utilizes coating agents, bovine serum albumin, create consistent rotation vibration individual treatment with agents intensifies torque induced while reducing friction at cell-cell cell-substrate interfaces, resulting Such can be modulated by varying frequency voltage applied sinusoidal AC signal achieved absence presence flow. enables survival functioning moving large-scale clusters investigated.