In vitro analysis of the interaction between yeast fimbrin and actin: A model for a mechanism of allele-specific suppression
作者： Tanya Marie Sandrock
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摘要: 11 CHAPTER ONE: Introduction 13 PART I: to the Cytoskeleton Actin Localization in Yeast 14 Identification of Proteins That Genetically Interact with 15 Fimbrin, Sac6p 16 II: Allele-Specific Suppression 19 Molecular Mechanisms High Copy 24 Reciprocal 25 Cycling-Suppressor Screens 26 Defining Domains 27 Dominant vs. Recessive Suppressors 28 Null 29 Intergenic Noncomplementation Intragenic 30 Other Examples 31 Techniques Concluding Remarks 32 TWO: Materials and Methods 35 Purification Protein Sequence Analysis 36 CNBr Cleavage for Sequencing 37 Trypsin Digestion 38 Bmding Bundling Assays Calcium Experiment Anti-Sac6p /uitibody 39 Gel Filtration 40
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