Diversity analysis of sulfite‐ and sulfate‐reducing microorganisms by multiplex dsrA and dsrB amplicon sequencing using new primers and mock community‐optimized bioinformatics

摘要: Genes encoding dissimilatory sulfite reductase (DsrAB) are commonly used as diagnostic markers in ecological studies of sulfite‐and sulfate‐reducing microorganisms. Here, we developed new high‐coverage primer sets for generation of reductive bacterial‐type dsrA and dsrB polymerase chain reaction (PCR) products for highly parallel amplicon sequencing and a bioinformatics workflow for processing and taxonomic classification of short dsrA and dsrB reads. We employed two diverse mock communities that consisted of 45 or 90 known …