3,4-Dideoxyglucosone-3-ene (3,4-DGE): A cytotoxic glucose degradation product in fluids for peritoneal dialysis

摘要: 3,4-Dideoxyglucosone-3-ene (3,4-DGE): A cytotoxic glucose degradation product in fluids for peritoneal dialysis. Background Bioincompatible products (GDPs) dialysis (PD) develop during sterilization and storage. Their biological activity has successfully been monitored through the use of various vitro methods but their molecular chemical nature is less well understood. Many GDPs are highly reactive carbonyl compounds. Although some identified extremely cytotoxic, none them actually possess cytotoxicity at concentrations found PD fluids. Thus, GDP responsible toxicity not yet identified. The intention present work was to investigate what extent unsaturated dicarbonyl compound, 3,4-dideoxyglucosone-3-ene (3,4-DGE) fluids, if it could be effects on L-929 fibroblast cells. Methods commercial preparation 3,4-DGE two different liquid chromatography were used identification quantification. In bioincompatibility determined as inhibition cell growth using line. Results conventionally manufactured a concentration 9 22 μmol/L. newly developed fluid, Gambrosol trio, 0.3 0.7 When added synthetic media measured conventional significantly lower than that seen with However, total amount available toxic reactions most probably higher measured, because freshly recruited from pool when consumed. speed this recruitment high enough explain heat-sterilized Conclusion between μmol/L, biologically active all date. main candidate held clinical caused by