Endothelial cell behavior inside myoblast sheets with different thickness
作者： Trung Xuan Ngo , Eiji Nagamori , Tetsutaro Kikuchi , Tatsuya Shimizu , Teruo Okano
DOI: 10.1007/S10529-013-1174-X
关键词:
摘要: Using a cell sheet stacking method, we developed an in vitro culture system which green fluorescent protein expressing human umbilical vein endothelial cells (GFP-HUVECs) were cultured under skeletal muscle myoblast (HSMM) sheets with different layer numbers. Our aim developing this was to examine the behaviors sheet. During 96 h of incubation, monolayer HSMM sheet, HUVECs quickly reached top and detached. In three-layered also migrated formed island-shaped aggregates. five-layered into middle net-shaped seven-layered basal sparse The thickness can be controlled by number is therefore important parameter that affects migration time, encounters, localization, morphology inside
springer.com
sci-hub.se 参考文章(16)
Maitane Perez-Ilzarbe, Onnik Agbulut, Beatriz Pelacho, Cristina Ciorba, Edurne San Jose-Eneriz, Michel Desnos, Albert A. Hagège, Pablo Aranda, Enrique J. Andreu, Philippe Menasché, Felipe Prósper, Characterization of the paracrine effects of human skeletal myoblasts transplanted in infarcted myocardium. European Journal of Heart Failure. ,vol. 10, pp. 1065- 1072 ,(2008) , 10.1016/J.EJHEART.2008.08.002
Rutger J Hassink, Aart Brutel de la Rivière, Christine L Mummery, Pieter A Doevendans, Transplantation of cells for cardiac repair. Journal of the American College of Cardiology. ,vol. 41, pp. 711- 717 ,(2003) , 10.1016/S0735-1097(02)02933-9
Shiplu Roy Chowdhury, Yuichi Muneyuki, Yasunori Takezawa, Masahiro Kino-oka, Atsuhiro Saito, Yoshiki Sawa, Masahito Taya, None, Growth and differentiation potentials in confluent state of culture of human skeletal muscle myoblasts Journal of Bioscience and Bioengineering. ,vol. 109, pp. 310- 313 ,(2010) , 10.1016/J.JBIOSC.2009.09.042
Sachiko Sekiya, Megumi Muraoka, Tadashi Sasagawa, Tatsuya Shimizu, Masayuki Yamato, Teruo Okano, Three-dimensional cell-dense constructs containing endothelial cell-networks are an effective tool for in vivo and in vitro vascular biology research. Microvascular Research. ,vol. 80, pp. 549- 551 ,(2010) , 10.1016/J.MVR.2010.08.001
Naosumi Sekiya, Goro Matsumiya, Shigeru Miyagawa, Atsuhiro Saito, Tatsuya Shimizu, Teruo Okano, Naomasa Kawaguchi, Nariaki Matsuura, Yoshiki Sawa, Layered implantation of myoblast sheets attenuates adverse cardiac remodeling of the infarcted heart. The Journal of Thoracic and Cardiovascular Surgery. ,vol. 138, pp. 985- 993 ,(2009) , 10.1016/J.JTCVS.2009.02.004
Masahiro Kino-oka, Trung Xuan Ngo, Eiji Nagamori, Yasunori Takezawa, Yasuki Miyake, Yoshiki Sawa, Atsuhiro Saito, Tatsuya Shimizu, Teruo Okano, Masahito Taya, Evaluation of vertical cell fluidity in a multilayered sheet of skeletal myoblasts Journal of Bioscience and Bioengineering. ,vol. 113, pp. 128- 131 ,(2012) , 10.1016/J.JBIOSC.2011.09.001
Nahoko Asakawa, Tatsuya Shimizu, Yukiko Tsuda, Sachiko Sekiya, Tadashi Sasagawa, Masayuki Yamato, Fumio Fukai, Teruo Okano, Pre-vascularization of in vitro three-dimensional tissues created by cell sheet engineering. Biomaterials. ,vol. 31, pp. 3903- 3909 ,(2010) , 10.1016/J.BIOMATERIALS.2010.01.105
Eiji Nagamori, Trung Xuan Ngo, Yasunori Takezawa, Atsuhiro Saito, Yoshiki Sawa, Tatsuya Shimizu, Teruo Okano, Masahito Taya, Masahiro Kino-oka, Network formation through active migration of human vascular endothelial cells in a multilayered skeletal myoblast sheet Biomaterials. ,vol. 34, pp. 662- 668 ,(2013) , 10.1016/J.BIOMATERIALS.2012.08.055
William Wood, Paul Martin, Structures in focus--filopodia. The International Journal of Biochemistry & Cell Biology. ,vol. 34, pp. 726- 730 ,(2002) , 10.1016/S1357-2725(01)00172-8
Shigeru Miyagawa, Matthias Roth, Atsuhiro Saito, Yoshiki Sawa, Sawa Kostin, Tissue-Engineered Cardiac Constructs for Cardiac Repair The Annals of Thoracic Surgery. ,vol. 91, pp. 320- 329 ,(2011) , 10.1016/J.ATHORACSUR.2010.09.080