Serum-Free Virus Propagation Platform For A Virus Vaccine Candidate
作者： Misa Sugui , Wenlin Zeng , Inn Yuk
DOI:
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摘要: The invention relates to methods for propagating viruses. In particular, the provides optimized conditions Optimization of following parameters are provided: lipid concentrates as supplements medium, temperature shift from pre-infection post-infection, multiplicity infection, direct bead-to-bead transfer and serum supplementation medium. first time a method virus by culturing cells that infected with in medium comprising chemically defined concentrate (CDLC). another claim, CDLC is added substantially free culture virus-infected cells.
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