Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine development

摘要: Enterovirus 71 (EV71) is an enterovirus that could lead to severe neurological disorders and fatalities. The inactivated vaccine appropriate EV71 format for meeting current needs. Large-scale preparation of the EV71vaccine depends on a scalable cell culture system industrial mass production. In this paper, Vero cells were found produce higher titers than did MRC-5 WI-38 cells. High-density microcarrier cultures established using 5g/L Cytodex 1 microcarriers promote release EV71s from infected For large-scale production antigen, extracellular virus produced in 2L bioreactor be 10 times lower spinner flask but improved by 30-folds glucose/glutamine feedings during infection. A serum-free was also bioreactor, yielding high-titer 5.8 x 10(7) TCID50/mL immunogenicity virions elicited slightly level neutralizing antibody response immunized mice. These results constitute valuable information development process producing vaccine.