Biopreservation of red blood cells--the struggle with hemoglobin oxidation.

摘要: One of the least recognized causes cellular damage during ex vivo preservation red blood cells is oxidative injury to hemoglobin. The latter has been associated with hemolysis through release toxic substances and oxidation vital cell components. This review delineates some major pathways that link hemoglobin damage, summarizes incidence hypothermic storage, cryopreservation desiccation stress. Red despite its success, not exempt from injury. Growing evidence portrays a time-dependant assault including formation reactive oxygen species, attachment denatured membrane phospholipids hemoglobin-containing microvesicles throughout storage. Similar symptoms have observed in attempts stabilize dried state, which methemoglobin levels reconstituted reached 50%. Factors affecting rate storage include compromised antioxidant activity, high concentrations glucose media presence molecular oxygen. Hemoglobin largely dictates our ability effectively preserve cells. Understanding origins along investigating methods minimize it can significantly improve quality future products.