A GCN2-Like eIF2α Kinase (LdeK1) of Leishmania donovani and Its Possible Role in Stress Response

摘要: Translation regulation in Leishmania parasites assumes significance particularly because they encounter myriad of stresses during their life cycle. The eukaryotic initiation factor 2α (eIF2α) kinases, the well-known regulators translation higher eukaryotes have now been found to control various processes these protozoan as well. Here, we report on cloning and characterization a GCN2-like eIF2α kinase from L. donovani also its modulation nutrient starvation. We cloned donovani, which named LdeK1 validated it be functional by vitro assay. was localized cytoplasm promastigotes with five-fold expression this stage parasite compared axenic amastigotes. Phosphorylation G1-arrest observed response starvation wild-type parasites. In contrast, phosphorylation significantly impaired dominant-negative mutant stress subsequent failure bring about cell Thus, is responds phosphorylating substrate, Nutrient encountered inside vector triggers metacyclogenesis. therefore propose that global translational activation followed gut sandfly could one mechanisms retool cellular machinery required for metacyclogenesis promastigotes.