High-throughput sequencing of frozen and paraffin-embedded tumor and normal tissue

摘要: Until now high-throughput sequencing of tumor samples relied on DNA isolated from fresh frozen tissues, the preparation which, however, is relatively laborious. The use preserved material, i.e. tissue banks, could help to avoid this limitation and would enable reanalysis diverse clinical trials. So far we have shown that formalin-fixed paraffin-embedded (FFPE) can be used for genomic re-sequencing processes. FFPE are amply available surgical resections histopathological diagnosis, comprise precursor lesions, primary tumors, lymphogenic and/or hematogenic metastases. To generate models which predict response therapy, also has advantage it a variety Second generation techniques not only applicable snap tissues whole genome analyses but targeted resequencing approaches. In addition, detection copy number variations mutations in obtained within one run. possibility using genome-wide technologies irrespective mode storage facilitates retrieval useful material prerequisite subsequent computational modelling