Absence of a Ku-like DNA end binding activity in the xrs double-strand DNA repair-deficient mutant.
作者： R C Getts , T D Stamato
DOI: 10.1016/S0021-9258(17)33960-1
关键词:
摘要: Double-strand DNA break repair is important in maintaining the genetic integrity of genome. Using a mobility shift assay, we find that protein, or complex proteins, present mammalian and yeast cells binds to ends double-strand renders resistant exonuclease digestion. Additionally, repair-deficient mutant, xrs, has no observable end binding activity, while revertant cell wild-type activity. In addition, supershift assays using monoclonal antibodies human Ku antigen (M(r) 70,000 subunit) reveal one proteins this activity may be protein with similar antigenic determinants. These observations suggest end-binding function repair.
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