Distribution of mRNA in the cytoplasmic polyribosomes of the HeLa cell.

作者: Harriet Latham , J.E. Darnell

DOI: 10.1016/S0022-2836(65)80224-8

关键词:

摘要: The cytoplasmic polyribosomes of HeLa cells have a broad spectrum sizes. larger structures contain the largest mRNA, while smallest mixture large and small mRNA. Evidence is presented that this distribution mRNA represents steady state in these many molecules are therefore incompletely loaded with ribosomes. transit time newly synthesized molecule into polysomes found to be less than seven minutes, implying little or no pool preformed destined enter polyribosomes.

参考文章(19)
Frederick L. Schaffer, Carlton E. Schwerdt, Purification and properties of poliovirus. Advances in Virus Research. ,vol. 6, pp. 159- 204 ,(1959) , 10.1016/S0065-3527(08)60491-1
W.K. Joklik, Y. Becker, Studies on the genesis of polyribosomes. I. Origin and significance of the subribosomal particles. Journal of Molecular Biology. ,vol. 13, pp. 496- 510 ,(1965) , 10.1016/S0022-2836(65)80112-7
Walter Gilbert, Polypeptide synthesis in Escherichia coli Journal of Molecular Biology. ,vol. 6, pp. 389- 403 ,(1963) , 10.1016/S0022-2836(63)80051-0
Robert G. Martin, Bruce N. Ames, A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures Journal of Biological Chemistry. ,vol. 236, pp. 1372- 1379 ,(1961) , 10.1016/S0021-9258(18)64180-8
Norman P. Salzman, Edwin D. Sebring, Utilization of precursors for nucleic acid synthesis by human cell cultures Archives of Biochemistry and Biophysics. ,vol. 84, pp. 143- 150 ,(1959) , 10.1016/0003-9861(59)90563-6
Alfred Gierer, Function of aggregated reticulocyte ribosomes in protein synthesis Journal of Molecular Biology. ,vol. 6, pp. 148- IN8 ,(1963) , 10.1016/S0022-2836(63)80131-X
S. Penman, Y. Becker, J.E. Darnell, A cytoplasmic structure involved in the synthesis and assembly of poliovirus components Journal of Molecular Biology. ,vol. 8, pp. 541- 555 ,(1964) , 10.1016/S0022-2836(64)80010-3
E.T. Bolton, B.J. McCarthy, Fractionation of complementary RNA Journal of Molecular Biology. ,vol. 8, pp. 201- 209 ,(1964) , 10.1016/S0022-2836(64)80129-7
E. Reich, R. M. Franklin, A. J. Shatkin, E. L. Tatum, Effect of actinomycin D on cellular nucleic acid synthesis and virus production. Science. ,vol. 134, pp. 556- 557 ,(1961) , 10.1126/SCIENCE.134.3478.556
A. Rich, S. Penman, Y. Becker, J. Darnell, C. Hall, Polyribosomes: Size in Normal and Polio-Infected HeLa Cells Science. ,vol. 142, pp. 1658- 1663 ,(1963) , 10.1126/SCIENCE.142.3600.1658