Bioremediation of petroleum-contaminated soil by a combined system of biostimulation-bioaugmentation with yeast

摘要: This paper presents a study of the effect combined biostimulation–bioaugmentation treatment applied to clay-loam soil contaminated with 16,300 mg/kg total petroleum hydrocarbons (TPH), which comprised 51% saturated and 31% aromatic hydrocarbons. The bioaugmentation was performed yeast Candida tropicalis SK21 isolated from petroleum-contaminated soil. strain able grow in pH range 3–9 liquid culture, optimum found be 6 for both growth biosurfactant production. At 6, 96% 42% TPH were degraded by at initial diesel oil concentrations 0.5% 5% (v/v), respectively. remediation via inoculating removed 83% 180 days while experiment indigenous microorganisms alone 61%. Microbial enumeration showed that could good It also dehydrogenase polyphenoloxidase activities remarkably enhanced inoculation ...