Analyses of isoamylase gene activity in wild-type barley indicate its involvement in starch synthesis.

摘要: The notion of debranching enzyme activity as a participant in starch synthesis is gaining acceptance. Inconsistent reports from mutant analyses implicate either isoamylase or pullulanase determinant amylopectin formation and whether wild-type plants utilize one the other, both, these enzymes unclear. Recent results on su1 maize suggest that both forms might be involved formation. We wished to find out if takes part by comparing gene under three conditions: (1) during accumulation developing sink tissues; (2) degradation germinating seeds; (3) ectopic expression after applying sucrose, precursor. isolated for barley isoamylase, iso1, analysed its regulation seeds, endosperm vegetative tissues, compared tissues different species. Our indicate modulated sucrose.