Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii.

摘要: In Pleurotus sp., green mold, which is considered a major epidemic, caused by several Trichoderma species. To develop rapid molecular marker specific for spp. that potentially cause eleven species were collected from mushroom farms and the Korean Agricultural Culture Collection (KACC). A dominant fungal isolate mold-infected substrate was identified as pleuroticola based on sequences of its internal transcribed spacer (ITS) translation elongation factor 1-α (tef1) genes. artificial inoculation tests, all spp., including T. atroviride, cf. virens, citrinoviride, harzianum, koningii, longibrachiatum, pleurotum, pleuroticola, showed pathogenicity to some extent, observed symptoms soaked mycelia with red-brown pigment retarded mycelium regeneration. developed detection wide range DNA sequence alignment ITS1 ITS2 regions The primer set detected only no cross reactivity edible mushrooms observed. limits PCR assay harzianum (KACC40558), pleurotum (KACC44537), (CAF-TP3) found be 500, 50, 5 fg, respectively, limit pathogen-to-host ratio approximately 1:10,000 (wt/wt).