Characterization of the Metabolic Response of Streptomyces clavuligerus to Shear Stress in Stirred Tanks and Single-Use 2D Rocking Motion Bioreactors for Clavulanic Acid Production.

摘要: Streptomyces clavuligerus is a gram-positive filamentous bacterium notable for producing clavulanic acid (CA), an inhibitor of β-lactamase enzymes, which confers resistance to bacteria against several antibiotics. Here we present comparative analysis the morphological and metabolic response S. linked CA production under low high shear stress conditions in 2D rocking-motion single-use bioreactor (CELL-tainer ®) stirred tank (STR), respectively. The CELL-tainer® guarantees turbulence enhanced volumetric mass transfer at stress, (in contrast bubble columns) allows investigation impact without oxygen limitation. results indicate that forces do not compromise viability cells; even higher specific growth rate, biomass, rate were observed STR. Under mycelial diameter increased considerably (average 2.27 vs. 1.44 µm STR). This suggests may be affected by lower surface-to-volume ratio would lead diffusion transport nutrients, oxygen, product. study shows there strong correlation between macromorphology production, should important aspect consider industrial CA.