ROS-induced HSP70 promotes cytoplasmic translocation of high-mobility group box 1b and stimulates antiviral autophagy in grass carp kidney cells.

摘要: Autophagy plays many physiological and pathophysiological roles. However, the roles regulatory mechanisms of autophagy in response to viral infections are poorly defined teleost fish, such as grass carp (Ctenopharyngodon idella), which is one most important aquaculture species China. In this study, we found that both reovirus (GCRV) infection hydrogen peroxide (H2O2) treatment induced accumulation reactive oxygen (ROS) C. idella kidney cells stimulate autophagy. Suppressing ROS with N-acetyl-l-cysteine significantly inhibited GCRV-induced activation enhanced GCRV replication. Although ROS-induced autophagy, turn, restricted replication, further investigation revealed multifunctional cellular protein high-mobility group box 1b (HMGB1b) serves a heat shock 70 (HSP70)–dependent, pro-autophagic carp. Upon H2O2 treatment, cytoplasmic HSP70 translocated nucleus, where it interacted HMGB1b promoted translocation HMGB1b. Overexpression siRNA-mediated knockdown assays indicated synergistically enhance autophagic cytoplasm. Moreover, reinforced an association ortholog Beclin 1 (a mammalian autophagy-associated yeast ATG6) by directly interacting 1. summary, study highlights antiviral function reveals positive role HMGB1b-mediated initiation fish.