Cloning and sequencing of cDNA of Sarcophaga peregrina humoral lectin induced on injury of the body wall.

作者: H Takahashi , H Komano , N Kawaguchi , N Kitamura , S Nakanishi

DOI: 10.1016/S0021-9258(17)39014-2

关键词:

摘要: A previous paper described the purification of a lectin induced in hemolymph larvae Sarcophaga peregrina (flesh-fly) on injury their body wall (Komano, H., Mizuno, D., and Natori, S. (1980) J. Biol. Chem. 255, 2919-2924). This describes cDNA cloning complete nucleotide sequence gene for lectin. Although active consists alpha beta subunits molar ratio 2:1, fat injured was found to contain only mRNA subunit, suggesting that these two are derived from common subunit is converted post-translationally. The consist 260 amino acid residues with an additional signal 19 or 23 residues.

参考文章(24)
H. Komano, D. Mizuno, S. Natori, A possible mechanism of induction of insect lectin. Journal of Biological Chemistry. ,vol. 256, pp. 7087- 7089 ,(1981) , 10.1016/S0021-9258(19)68925-8
H. Komano, D. Mizuno, S. Natori, Purification of lectin induced in the hemolymph of Sarcophaga peregrina larvae on injury. Journal of Biological Chemistry. ,vol. 255, pp. 2919- 2924 ,(1980) , 10.1016/S0021-9258(19)85828-3
D.A. Morrison, [21] Transformation and preservation of competent bacterial cells by freezing Methods in Enzymology. ,vol. 68, pp. 326- 331 ,(1979) , 10.1016/0076-6879(79)68023-0
Allan M. Maxam, Walter Gilbert, [57] Sequencing end-labeled DNA with base-specific chemical cleavages Nucleic Acids Part I. ,vol. 65, pp. 499- 560 ,(1980) , 10.1016/S0076-6879(80)65059-9
H Komano, R Nozawa, D Mizuno, S Natori, Measurement of Sarcophaga peregrina lectin under various physiological conditions by radioimmunoassay. Journal of Biological Chemistry. ,vol. 258, pp. 2143- 2147 ,(1983) , 10.1016/S0021-9258(18)32899-0
Hugh R. B. PELHAM, Richard J. JACKSON, An efficient mRNA-dependent translation system from reticulocyte lysates. FEBS Journal. ,vol. 67, pp. 247- 256 ,(1976) , 10.1111/J.1432-1033.1976.TB10656.X
Jack Kyte, Russell F. Doolittle, A simple method for displaying the hydropathic character of a protein Journal of Molecular Biology. ,vol. 157, pp. 105- 132 ,(1982) , 10.1016/0022-2836(82)90515-0
Shunji Natori, Haruo Takahashi, Hiroto Komano, Nobuaki Kawaguchi, Masuo Obinata, Activation of the secretion of specific proteins from fat body following injury to the body wall of Sarcophaga peregrina larvae Insect Biochemistry. ,vol. 14, pp. 713- 717 ,(1984) , 10.1016/0020-1790(84)90051-9