The benefits and limitations of reaction cell and sector field inductively coupled plasma mass spectrometry in the detection and quantification of phosphopeptides
作者： Evelyne Maes , Wilfried Brusten , Filip Beutels , Geert Baggerman , Inge Mertens
DOI: 10.1002/RCM.7079
关键词:
摘要: RATIONALE The phosphorylation of proteins is one the most important post-translational modifications in nature. Knowledge quantity or degree protein biological samples extremely important. A combination liquid chromatography (LC) and inductively coupled plasma mass spectrometry (ICP-MS) allows absolute relative quantification phosphorus signal. METHODS A comparison between dynamic reaction cell quadrupole ICP-MS (DRC-Q-ICP-MS) high-resolution sector field (SF-ICP-MS) detecting signals phosphorus-containing species using identical capillary LC (reversed-phase technology) nebulizer settings was performed. RESULTS A method to diminish reversed-phase gradient-related signal instability detection with LC/ICP-MS applications developed. Bis(4-nitrophenyl)phosphate (BNPP) used as a standard compare signal-to-noise ratios limits (LODs) two instrumental setups. The LOD reaches value 0.8 µg L−1 when applying DRC technology Q-ICP-MS an 0.09 µg L−1 found SF-ICP-MS setup. This BNPP further possibilities phosphopeptides these setups. CONCLUSIONS This one-to-one interference-reducing instruments demonstrates that individual LC-separated possible. However, based on values, has higher sensitivity thus preferred phosphopeptide analysis. Copyright © 2014 John Wiley & Sons, Ltd.
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