Contrast-enhanced ultrasound detects perfusion defects in an ex vivo porcine liver model: a useful tool for the study of hepatic reperfusion
作者： Ahmed Alzaraa , Dhya Al-Leswas , Wen Yuan Chung , Gianpiero Gravante , Morgan Bruno
DOI: 10.1007/S10047-013-0717-1
关键词:
摘要: Following transplantation, areas of hypoperfusion can be associated with metabolic changes and poor organ recovery. Our study evaluated contrast-enhanced ultrasound (CEUS) agents for the detection such areas. Livers were collected from ten pigs, connected to extracorporeal circuits perfused using autologous blood. After 1 4 h livers scanned an machine following administration CEUS agents. Biopsies non-perfused collected. The entire parenchyma enhanced strongly on non-contrast at 1 h no perfusion defects. Four hours later multiple defects manifested not evident ultrasound. Histology confirmed corresponded ischemic zones. In our model addition revealed after 4 h. This might facilitate characterization in transplanted livers.
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A Nistri, M Angelico, C Casciani, A Anselmo, L Baiocchi, G Tisone, C Gandin, F Pisani, Sequential changes in serum and biliary bile acids after liver transplantation. International Pancreas and Islet Transplant Association. Congress. ,vol. 27, pp. 3503- 3504 ,(1995)
A Nistri, M Angelico, A Anselmo, L Baiocchi, G Tisone, C Gandin, J Romagnoli, C Umberto Casciani, F Pisani, Patterns of bile salts and biliary lipids early after liver transplantation differentiate patients with unfavorable graft outcome. International Congress of the Asian Transplantation Surgery. ,vol. 28, pp. 1655- 1656 ,(1996)
Shushma Aggarwal, Walter Obrist, Howard Yonas, David Kramer, Yoogoo Kang, Victor Scott, Raymond Planinsic, Cerebral hemodynamic and metabolic profiles in fulminant hepatic failure: relationship to outcome. Liver Transplantation. ,vol. 11, pp. 1353- 1360 ,(2005) , 10.1002/LT.20479
D.-A. Clevert, M. Stickel, N. Minaifar, F. Löhe, C. Graeb, K.W. Jauch, M. Reiser, Contrast-enhanced ultrasound in liver transplant: First results and potential for complications in the postoperative period Clinical Hemorheology and Microcirculation. ,vol. 43, pp. 83- 94 ,(2009) , 10.3233/CH-2009-1223
Gianpiero Gravante, Seok Ling Ong, Matthew S. Metcalfe, Roberto Sorge, John Overton, David M. Lloyd, Guy J. Maddern, Ashley R. Dennison, Cytokine response of electrolytic ablation in an ex vivo perfused liver model Anz Journal of Surgery. ,vol. 80, pp. 537- 541 ,(2010) , 10.1111/J.1445-2197.2010.05380.X
Ahmed Alzaraa, Gianpiero Gravante, Wen Yuan Chung, Dhya Al-Leswas, Morgan Bruno, Ashley R. Dennison, David M. Lloyd, Targeted microbubbles in the experimental and clinical setting. American Journal of Surgery. ,vol. 204, pp. 355- 366 ,(2012) , 10.1016/J.AMJSURG.2011.10.024
Robert C.G. Martin, Nathan P. Reuter, Charles Woodall, Intra-operative contrast-enhanced ultrasound improves image enhancement in the evaluation of liver tumors. Journal of Surgical Oncology. ,vol. 101, pp. 370- 375 ,(2010) , 10.1002/JSO.21511
Jonathan R. Lindner, Microbubbles in medical imaging: current applications and future directions. Nature Reviews Drug Discovery. ,vol. 3, pp. 527- 532 ,(2004) , 10.1038/NRD1417
Jie Ren, Ming-De Lu, Rong-Qin Zheng, Min-Qiang Lu, Mei Liao, Yong-Jiang Mao, Zhi-Juan Zheng, Yan Lu, Evaluation of the microcirculatory disturbance of biliary ischemia after liver transplantation with contrast-enhanced ultrasound: Preliminary experience Liver Transplantation. ,vol. 15, pp. 1703- 1708 ,(2009) , 10.1002/LT.21910
Wen Yuan Chung, Gianpiero Gravante, Dhya Al-Leswas, Ahmed Alzaraa, Roberto Sorge, Seok Ling Ong, Cristina Pollard, David M. Lloyd, Matthew S. Metcalfe, Ashley R. Dennison, Addition of a kidney to the normothermic ex vivo perfused porcine liver model does not increase cytokine response Journal of Artificial Organs. ,vol. 15, pp. 290- 294 ,(2012) , 10.1007/S10047-012-0641-9