Closely Spaced Tandem Arrangement of AQP2, AQP5, and AQP6 Genes in a 27-Kilobase Segment at Chromosome Locus 12q13

摘要: Abstract The aquaporins (AQPs) are a family of water-transporting proteins that facilitate osmotically driven water movement across cell plasma membranes. Among the seven human cloned to date (AQPs 0–6), genes encoding four most closely related (AQP0, AQP2, AQP5, and AQP6) have been mapped chromosome band 12q13, suggesting an aquaporin gene cluster at this locus. To construct physical map identify novel members on cluster, CEPH B yeast artificial (YAC) library was screened by PCR using primers derived from exon 4 AQP2 AQP0 genes. A YAC clone with 200 kb DNA isolated analyzed. Primary pulsed-field gel electrophoresis Southern blot analysis indicated presence AQP6 genes, but not AQP0. Restriction mapping yielded precise in which three span only ∼27 order, transcriptional orientation, spacer length 5′-AQP2–5 spacer–AQP5–7 spacer–AQP6-3′.