Comparison of Wheat Germ Agglutinin‐ and Phorbol Myristate Acetate‐Mediated Triggering for Macrophage H2O2 Release

摘要: Murine peritoneal macrophages (M phi S) elicited by a single injection of zymosan A showed higher responsiveness to the wheat germ agglutinin (WGA)-mediated triggering for H2O2 release than M S activated double injections agent. On contrary, response phorbol myristate acetate(PMA)-mediated was in latter case former S. Furthermore, PMA-triggered found be inhibited sarcoma 180 tumor cell-derived proteinaceous factor much more marked fashion WGA-triggered release. These results indicate some significant differences between cellular mechanisms WGA- and PMA-triggering oxidative burst. other hand, microfilament-inhibitors (cytochalasins B E) serine protease-inhibitors (tosyl-L-lysine-chloromethyl ketone tosylamido-2-phenylethyl-chloromethyl ketone), but not microtubule-disrupting agents (colchicine, vinblastine, vincristine), suppressed both PMA-triggerings similar degree, thereby indicating that burst have common process which is dependent on microfilament protease functions. In relation this, spreading were also depressed microfilament- microtubule-inhibitors, participation membrane functions signal transduction cases burst, cell induced as well PMA-triggering.