Genotyping of type b Haemophilus influenzae strains, comparison of strains collected before and during vaccine availability.

摘要: Abstract Objective and method. – Five hundred seventy-eight strains of type b Haemophilus influenzae (521 isolated in children, 57 adults) were compared using pulsed-field gel electrophoresis (PFGE) to assess strain evolution study the impact generalization anti- (anti-Hib) vaccination France. Among these strains, 398 (including 342 from meningitis) 1985–1992 (pre-vaccination era), 39 31 1993 (year anti-Hib vaccination), 141 50 CSF) 1994–2001 (vaccination era). Results. A total 102 PFGE patterns (patterns for 1–101 isolates) obtained after Sma I restriction 578 strains. The children distributed 96 patterns, those adult 34 patterns. during pre-vaccination era presented 94 During era, 50% disappeared 12 new (11.7%) including 15 observed. belonging two observed 1993) adults ( n  = 7) blood culture bronchial secretions,  = 9) CSF, culture, secretions. In among associated failure, with a pulsotype. Conclusion. There is no evidence that program brought about any drastic modifications causing meningitis or other circulation whether children.