MicroRNA 145 enhances chemosensitivity of glioblastoma stem cells to demethoxycurcumin.
作者： Chunfa Qian , Bin Wang , Yuanjie Zou , Yansong Zhang , Xinhua Hu
DOI: 10.2147/CMAR.S210076
关键词:
摘要: Background: The presence of glioma stem cells (GSCs) is thought to be a key factor responsible for development the incurable glioblastoma multiforme (GBM). GSCs are often displayed during chemotherapy resistance, except demethoxycurcumin (DMC), component curcumin, which has been previously confirmed inhibit proliferation and induce apoptosis. Purpose: objective this study was identify main mechanism underlying anti-GSCs resistance by DMC. Patients methods: qRT-PCR used determine expression miR-145 in patients GSCs, were transfected with overexpressed vectors. Then, functional analyses (in vitro vivo) performed confirm role DMC GSCs. Finally, related proteins tested immunohistochemistry Western blot. Results: atypically low-expressed miRNA could enhance GSC chemosensitivity both vivo. Upregulation resulted increased cell growth inhibition apoptosis Further research on demonstrated that combined effects involved miR-145/SOX2-Wnt/β-catenin pathway. Overexpression SOX2 reduced miR-145+ DMC treatment. Conclusion: Our data strongly support an important enhancing targeting SOX2-Wnt/β-catenin axis.
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