Cyclic ADP-ribose requires CD38 to regulate the release of ATP in visceral smooth muscle

摘要: It is well established that the intracellular second messenger cADP-ribose (cADPR) activates Ca2+ release from sarcoplasmic reticulum through ryanodine receptors. CD38 a multifunctional enzyme involved in formation of cADPR mammals. has also been reported to transport several cell lines. Here, we demonstrate role for extracellular and modulating spontaneous, but not electrical field stimulation-evoked, ATP visceral smooth muscle. Using small-volume superfusion assay an HPLC technique with fluorescence detection, measured spontaneous evoked bladder detrusor muscles isolated CD38+/+ CD38−/− mice. (1 nm) enhanced overflow bladders This effect was abolished by inhibitor receptors on 8-bromo-cADPR (80 μm) (50 μm), nonselective P2 purinergic receptor antagonist pyridoxal phosphate 6-azophenyl-2′,4′-disulfonate (30 μm). failed facilitate mice, indicating crucial enhancing effects release. Contractile responses were potentiated cADPR, suggesting two adenine nucleotides may work synergy maintain resting tone bladder. In conclusion, enhances influx via subsequent activation receptors, probably causing increase neuronal cells.