Effect of the Thawing Process on Cryopreserved Arteries
作者： Gemma Pascual , Natalio García-Honduvilla , Marta Rodríguez , Fernando Turégano , Julia Bujan
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摘要: This study was designed to explore the changes that occur in cryopreserved pig arteries following different thawing procedures, before and after being placed an vitro flow circuit. Segments of minipig iliac artery were complete minimal essential medium plus 10% dimethylsulphoxide stored liquid nitrogen at −196°C for 30 days. Three groups established according whether arterial specimens fresh (control, n = 20), rapidly thawed (RT) 37°C (n 22) or subjected controlled, automated slow (ST) 22). Half each group subsequently circuit 72 hr. Evaluation made morphological ultrastructural changes. Cell damage using TUNEL method. All showed endothelial denudation most extensive those rapid thawing. Slowly improved cell viability organization vessel wall, compared rapidly. Under conditions flow, induced by freezing/thawing process enhanced. These findings suggest (a) results preservation structure vessels, (b) is enhanced when vessels are
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C.J. Hunt, Y.C. Song, E.A.J. Bateson, D.E. Pegg, Fractures in cryopreserved arteries. Cryobiology. ,vol. 31, pp. 506- 515 ,(1994) , 10.1006/CRYO.1994.1061
Montserrat Rigol, Magda Heras, Anna Martínez, María Jesús Zurbano, Elba Agustí, Eulàlia Roig, José Luís Pomar, Ginés Sanz, Changes in the cooling rate and medium improve the vascular function in cryopreserved porcine femoral arteries. Journal of Vascular Surgery. ,vol. 31, pp. 1018- 1025 ,(2000) , 10.1067/MVA.2000.103793
Manfred Vischjager, Thomas M. Van Gulik, Jan Van Marle, Martin Pfaffendorf, Michael J.H.M. Jacobs, Function of cryopreserved arterial allografts under immunosuppressive protection with cyclosporine A Journal of Vascular Surgery. ,vol. 24, pp. 876- 882 ,(1996) , 10.1016/S0741-5214(96)70026-5
Eugenio Rosset, Alain Friggi, Gisèle Novakovitch, Pierre-Henri Rolland, Regis Rieu, Jean-François Pellissier, Pierre-Edouard Magnan, Alain Branchereau, Effects of cryopreservation on the viscoelastic properties of human arteries. Annals of Vascular Surgery. ,vol. 10, pp. 262- 272 ,(1996) , 10.1007/BF02001892
David E. Pegg, Monica C. Wusteman, Serena Boylan, Fractures in cryopreserved elastic arteries Cryobiology. ,vol. 34, pp. 183- 192 ,(1997) , 10.1006/CRYO.1996.1997
Frank J. Veith, Sushil K. Gupta, Enrico Ascer, Sheila White-Flores, Russell H. Samson, Larry A. Scher, Jonathan B. Towne, Victor M. Bernhard, Patricia Bonier, William R. Flinn, Patricia Astelford, James S.T. Yao, John J. Bergan, Six-year prospective multicenter randomized comparison of autologous saphenous vein and expanded polytetrafluoroethylene grafts in infrainguinal arterial reconstructions Journal of Vascular Surgery. ,vol. 3, pp. 104- 114 ,(1986) , 10.1016/0741-5214(86)90073-X
A Negoescu, P Lorimier, F Labat-Moleur, C Drouet, C Robert, C Guillermet, C Brambilla, E Brambilla, In situ apoptotic cell labeling by the TUNEL method: improvement and evaluation on cell preparations. Journal of Histochemistry and Cytochemistry. ,vol. 44, pp. 959- 968 ,(1996) , 10.1177/44.9.8773561
E. Müller-Schweinitzer, M.J. Mihatsch, M. Schilling, W.E. Haefeli, Functional recovery of human mesenteric and coronary arteries after cryopreservation at −196° C in a serum-free medium Journal of Vascular Surgery. ,vol. 25, pp. 743- 750 ,(1997) , 10.1016/S0741-5214(97)70304-5
M Bennett, Apoptosis of vascular smooth muscle cells in vascular remodelling and atherosclerotic plaque rupture. Cardiovascular Research. ,vol. 41, pp. 361- 368 ,(1999) , 10.1016/S0008-6363(98)00212-0
Yves Castier, Guy Lesèche, Tonino Palombi, Marie-Dominique Petit, Olivier Cerceau, Early experience with cryopreserved arterial allografts in below-knee revascularization for limb salvage American Journal of Surgery. ,vol. 177, pp. 197- 202 ,(1999) , 10.1016/S0002-9610(99)00010-0