Calcium currents in striatal fast-spiking interneurons: dopaminergic modulation of CaV1 channels.

摘要: Striatal fast-spiking interneurons (FSI) are a subset of GABAergic cells that express calcium-binding protein parvalbumin (PV). They provide feed-forward inhibition to striatal projection neurons (SPNs), receive cortical, thalamic and dopaminergic inputs coupled together by electrical chemical synapses, being important components the circuitry. It is known dopamine (DA) depolarizes FSI via D1-class DA receptors, but no studies about ionic mechanism this action have been reported. Here we ask ion channels effectors actions. This work their Ca2+ currents. Whole-cell recordings in acutely dissociated identified from PV-Cre transgenic mice were used show an array voltage gated channel classes: CaV1, CaV2.1, CaV2.2, CaV2.3 CaV3. However, CaV1 carries most whole-cell current FSI. Activation D1-like class receptors D1-receptor selective agonist SKF-81297 (SKF) enhances currents through modulation. A previous block with nicardipine occludes DA-agonist, suggesting other modulated activation. Bath application SKF brain slices increases firing rate activity as measured both imaging recordings. These actions reduced nicardipine. The present discloses one final effector modulation We conclude facilitatory part due positive